The Problem of Assistance in Self

If the terminally ill parent focuses on his right and desire to die because the disease makes his life meaningless, it is possible to try to shift the parent’s attention from the quality-of-life perspective to the sanctity-of-life orientation in order to help him discuss the life as not meaningful or meaningless, but as the gift or a kind of blessing (Leming Dickinson, 2011, p. 262).Advertising We will write a custom critical writing sample on The Problem of Assistance in Self-Deliverance specifically for you for only $16.05 $11/page Learn More From this point, the child’s role in the parent’s self-deliverance should not be associated with any variant of euthanasia or assisted suicide. Thus, it is almost impossible for adult children to become willing to assist the parent in self-deliverance performed as a variant of the suicide. Those persons, who choose to focus on their right to die as one of the human rights, can be discussed as the followers of the quality-of-life perspective because stating that their life is not worth living, these persons concentrate on the quality of their daily feelings and sufferings (Leming Dickinson, 2011, p. 262). It is impossible for these persons to live a life full of sufferings because they cannot discuss this life as meaningful for them and for the society. The followers of such a philosophy could become the patients of Dr. Jack Kevorkian who assisted terminally ill patients in relieving their sufferings while committing the physician-assisted suicides (Murphy, 2011, p. 4). Kevorkian stated that he did not kill patients, but he performed the ‘suicide on demand’ which could relieve patients’ sufferings (Murphy, 2011, p. 4-5). The problem of the assisted suicide is closely associated with the problem of letting persons die. Nevertheless, the line between the acts of killing and letting die is subtle, and it is possible to state that the adult child who ass ists the parent in self-deliverance actually kills the terminally ill parent. The death becomes preferable to life when pain and sufferings cannot be born by people (Leming Dickinson, 2011, p. 262). The parent can focus on the death because of not seeing the perspectives in struggling any more.Advertising Looking for critical writing on social sciences? Let's see if we can help you! Get your first paper with 15% OFF Learn More However, it is important to pay attention to the fact that the parent can assess his quality of life only subjectively, while focusing on the moments and aspects which are important for him. To discourage the parent’s actions and intentions, it is necessary to tell the parent about the perspectives which are seen for the others because everyone’s life has the significant quality, and it is necessary to look at the point from many perspectives (Leming Dickinson, 2011, p. 264). This approach can work, if the parent feels the love of his family, and he can see the chance to relieve the pain. The other approach is the necessary focus on the sanctity-of-life perspective which can contribute to changing the parent’s decision more than the focus on the quality-of-life approach. The parent should remember that his life and sufferings have the great meaning while discussing the life as the divine gift. A human has no rights to decide about the moment of his death because the person’s obligation is to concentrate on protecting and prolonging the life (Leming Dickinson, 2011, p. 262). The final stage of the process developed to discourage the parent’s actions and decision is the demonstration of the importance of the parent’s life for all the family because of the parent’s focus on the quality of his living. References Leming, M., Dickinson, G. (2011). Understanding dying, death, and bereavement. Belmont, CA: Wadsworth Cengage Learning. Murphy, T. F. (2011). A philosophical obituary : Dr. Jack Kevorkian dead at 83 leaving end of life debate in the US forever changed. The American Journal of Bioethics, 11(7), 3-6.Advertising We will write a custom critical writing sample on The Problem of Assistance in Self-Deliverance specifically for you for only $16.05 $11/page Learn More This critical writing on The Problem of Assistance in Self-Deliverance was written and submitted by user Dark Wasp to help you with your own studies. You are free to use it for research and reference purposes in order to write your own paper; however, you must cite it accordingly. You can donate your paper here.

Dictatorship essays

Dictatorship essays In modern times, no dictator can take total power by force alone. In order to gain support, they must offer something beneficial to the people. Unfortunately what is thought to be beneficial can be extremely harmful and cruel. Three specific dictators during the World War II period were Mussolini, Hitler, and Stalin. Joseph Stalin was the successor of Lenin after his death. He had only one goal and that was to finish what Lenin had started. Basically that was to build a classless society in which the means of production were in the hands of the people. After Stalin established power, he developed new goals to make the Soviet Union a leader in industry. He wanted to get rid of Russias backwardness. For this reason Stalin proposed several five year plans. These plans were aimed at improving industry and economic growth. He developed a command economy, which meant government made all economic decisions. Under Stalin, the government controlled all businesses. Stalin also took agriculture under control of the government. He forced people to give up their privately owned land and live on government-owned farms or on large farms that were owned and operated by groups, also known as collectives. The state set all prices and controlled access to farm supplies. (Comptons Encyclopedia, 1990, Joseph Sta lin S570) On July 29, 1921, Adolf Hitler was introduced as Fuehrer of the Nazi Party. Fuehrer basically meant leader. By November 1923, the Nazis held approximately 55,000 followers and were the biggest and most powerful in Germany. The Nazi Party demanded action of Hitler. Hitler knew that he would lose his support if he didnt do something fast. So he and his party developed a plan to kidnap the leaders of the Bavarian government and force to accept Hitler as their leader. On November 9th, 1923 Hitler and his Nazis went to Munich and tried to take it over. At this point they were not powerful enough to ...

Report - Innovative team Management Plan Assignment

Report - Innovative team Management Plan - Assignment Example In addition, several global organizations focus on workplace diversity program to integrate the employees of different cultural background with the organizational culture and values. It is important for an organization to develop effective and improved workplace culture to maintain significant and hazard free business operations. Team Goals Leading organizations always try to develop effective team goals to meet organizational goals and objectives. (A) Specific team goals need to be developed by the leaders or managers as a significant part of team performance plan. Clear and shared team goals provide several advantages to the team performances. Effective team goals can help the managers to communicate with the team members about the specific role of the team. It can guide the team members in planning and development process. It helps the team members or employees within a small team to complete assigned tasks within a given period of time. Managers or leaders can easily evaluate the performance and outcome of a team work through these developed team goals. In addition, appropriate team goals can reduce the threat of group or team conflict within the workplace of an organization. (B) Several organizations implement various strategies to enhance creativity and innovation among the team within the workplace. Reward creativity, support creativity, workplace diversity and positive working environment are the four strategies that can enhance creativity and innovation among the team members. Effective reward distribution can motivate the skilled and talented employees to generate innovative ideas. It will help to complete the team project successfully. Support creativity strategy can help employees to take calculated risk in order to achieve significant project outcome. Workplace diversity will help to exchange different ideas among the employees. It can enhance the decision making process. Positive working environment can inspire the employees to perform well to ach ieve personal and professional objectives with greater flexibility. Innovation and Creativity Creativity is known as generation of several innovative ideas. On the other hand, innovation is the transformation of new ideas into a new organization, product, service or a process. (A) Several leading organizations are trying to differentiate their business operation through the implementation of innovative and creative business strategies. The demand for workplace creativity from the employees of an organization is rising in this age of technological revolution. Innovative leadership style can help an organization to change and implement new ideas and strategies in the workplace (Grivas and Puccio, 2011, p.123). Creative thinking and innovation are useful during the strategic planning process. Effective workplace diversity, work distribution and significant reward system are some examples of innovative and creative leadership style. (B) Several leading organizations are trying to implem ent new innovative management strategies in the workplace to achieve successful project output. Innovation teams, linking innovation with operation processes, and anonymity & confidentiality are

Bullfighting Essay Example | Topics and Well Written Essays - 500 words

Bullfighting - Essay Example After that the local public took it as a sport and since they could not afford it, they started bullfighting on bulls and the tradition still continues. Bullfights or corrida takes place in the presence of the president and the local audience in Spain. First of all the paseillo happens in which all the participants of the bullfight introduce themselves. Two participants called alguacilillos enter the ring and ask for keys to the gate behind which are the bulls. The doors are called puerta de los toriles. When the bulls come out the main show starts. The bullfights takes place in three parts called tercios. In the first part, the bull's ability to move his head is hampered by lances used by two picadors who are riding on blindfolded horses. In the second part, three people called banderilleros make the bull chase them around and use sharp colored sticks called banderillas and stab it in the body of the bull, specifically his back. Finally the third and the last part includes the killing of the bull by the matador. In my view, this is a very cruel sport and a symbol of inhuman behavior. Usually in this sport, the bulls are made weak by putting sand bags in their backs. They are also made weak by drugging the animal or to cut the horn of the bull which hamper coordination. Sometimes the weak and placid bulls are selected for the fight so that the win is easy. The game is not only cruel for the bull, but there are other victims as well.

Blastocystis Hominis and Colorectal Cancer

Blastocystis Hominis and Colorectal Cancer Blastocystis hominis (B.hominis) is the most common unicellular protozoan parasite that is found in the human gastrointestinal tract (Windsor et al. 2002). The prevalence of B. hominis in both developed and developing countries is reported to be up to 10% and 50% respectively (Wong et al. 2008; Stenzel and Boreham. 1996). On the other hand, B. hominis shows a diverse morphologies which include vacuolar, granular, amoeboid and cyst forms (Zierdt. 1988). Furthermore, B. hominis isolates from human and animals have been reported to exhibit an extensive genetic and karyotypic heterogeneity (Parkar et al. 2010; Stensvold et al. 2009; Tan et al. 2009; Dogruman-Al et al. 2009; Abe. 2004; Yoshikawa et al. 2004). Although many researchers have attempted to identify the pathogenesis of B. hominis in human hosts, however the pathogenic potential of B. hominis has remained controversial as it is present in both symptomatic and asymptomatic individual (Tan. 2008; Garcia. 2007; Tan et al. 2002). The gastrointestinal symptoms that are associated with B. hominis infection include diarrhea, vomiting, anorexia, flatulence, abdominal discomfort and other associated non-specific symptoms. To date, experiments have shown that rats that are inoculated with human B. hominis isolate have demonstrated an elevation in oxidative damage (Chandramathi et al. 2009). Many studies have been done on the genetic and molecular characterization of B. hominis isolates derived from human as well as animal. Recent molecular characterization of Blastocystis isolates suggested that there are 13 subtypes (ST1-ST13) which have been isolated from mammalian, reptilian, avian and amphibian hosts (U. Parkar et al. 2010; Stensvold et al. 2007a; Noà «l et al. 2005, 2003; Yoshikawa et al. 2004b). Recently, many studies have been done to determine the pathogenic potential of different Blastocystis subtypes in human hosts by genotypic analysis as well as molecular characterization of B. hominis isolated from symptomatic and asymptomatic individuals (Dominguez-Mà ¡rquez et al. 2009; Eroglu et al. 2009; Jones et al. 2009; Hussein et al. 2008; Tan et al. 2008). Nevertheless, only limited numbers of studies have shown correlation between the different B. hominis subtypes on the pathogenesis in human hosts (Hussein et al. 2008). Infectious agents such as bacteria, virus and parasites, often have an oncogenic potential. The International Agency on Research of Cancer (IARC) has estimated that 16% of cancer worldwide is caused by infection, including parasites. However, there has been no research done on the association between B. hominis infection and colorectal cancer. A recent study has suggested that B. hominis may possess the ability to induce the growth of colorectal cancer cell lines by inhibiting the apoptotic effect of colon cancer cells. Furthermore, the antigens that are isolated from B. hominis were postulated to be able to promote the proliferation of cancer cells via down-regulation of host immune cellular responses (Chandramathi et al. 2010). In general, inflammation is activated by a variety of stimuli such as trauma, bacterial/viral/parasitic infections, endotoxemia and heating (Ley, 2001; Hart, 2002). Inflammation caused by infectious agents such as parasites will cause an increase in the production of reactive oxygen species (ROS) such as nitric oxide, hydrogen peroxide and superoxide as a consequence of cell mediated phagocyte dependent immune response (Rosen et al. 1995). A persistent and chronic inflammatory response can be detrimental to human host as it can produce a chronic damage by releasing a variety of pro-inflammatory and anti-inflammatory cytokines which then leads to mutagenesis, carcinogenesis, neurodegenerative disorder, inflammatory bowel syndrome and atherosclerosis (Kà ¼hn et al. 1993; Perry et al. 1998; Ludewig et al. 2002; Shacter et al. 2002). Several studies have shown correlation between the inflammation that is caused by infectious agents such as parasites and the development of cancer in huma n (Fitzpatrick. 2001). Thus, it is important for us to evaluate the immunomodulation, cytopathic and cellular cytokines responses as a result of B. hominis infection especially in colorectal carcinomas. Since B. hominis is often present in most stool cultures, it is pertinent to investigate the association between B. hominis infection and the development of colorectal cancer in the gastrointestinal tract of infected host system. B. hominis isolated from an asymptomatic individual could facilitate the proliferation and growth of cancer cells and has the potential to down-regulate the host immune response (Chandramathi et al. 2010). However, only limited numbers of studies have shown the cytopathic effects and cellular cytokine responses of Blastocystis hominis infection (Puthia et al. 2008; Long et al. 2001; Walderich et al. 1998). These studies only investigated on Interleukin 8 and Granulocyte-macrophage colony stimulating factor (GM-CSF) which are pro-inflammatory cytokines. We hypothesized that B. hominis infection in human host system has a potential carcinogenic effect and could influence the growth of colorectal cancer cells especially in colorectal cancer patients and that the B. hominis subtypes may exert varying degree of pathogenecity. Therefore, in this study, the differences between the effects of solubilized antigen of B. hominis from both symptomatic and asymptomatic isolates on the cell viability in the peripheral blood mononuclear cells (PBMCs, which represent the immune cells) as well as colorectal cancer cell line was evaluated. Besides that, the gene expression of cytokine, apoptotic mediators, and nuclear transcriptional factors in response to the symptomatic and asymptomatic B. hominis antigen in both PBMCs and colorectal cancer cell was compared. The knowledge and understanding in the association between Blastocystis hominis infection and colorectal cancer as well as the role of various cytokines involved in the tumour progression may provide an insight into prevention and/or development of new immune-therapeutical strategies to combat colorectal cancer. OBJECTIVE The objectives of the present study are:- To study the effects of solubilized antigen of B. hominis from symptomatic and asymptomatic individual isolates on viability of both the peripheral blood mononuclear cells (PBMCs, which represent the normal immune cells) as well as colorectal cancer cells (HCT 116, colorectal cancer cell line). To investigate the gene expression of cytokine, transcriptional factors and apoptotic mediators such as interleukin 6, interleukin 8, tumour necrosis factor-ÃŽ ±, interferon gamma, nuclear factor kappa light chain enhancer of activated B cells, cathepsin B, transforming growth factor-ÃŽ ², and protein 53 upon exposure to B. hominis antigen in both the PBMCs and colorectal cancer cells MATERIALS AND METHODS Sample collection and axenization of Blastocystis hominis B. hominis was isolated from stool samples of symptomatic as well as asymptomatic individuals. The B. hominis cysts were then isolated from feacal sample using the Ficoll-Paque Technique according to Zaman and Khan (1994). The harvested cysts of B. hominis from both the symptomatic and asymptomatic individual isolates were washed in sterile phosphate-buffered saline (PBS). The washed harvested cysts were cultured in Jones medium, supplemented with 10% of heat-inactivated horse serum and incubated at 37 °C in CO2 incubator (Suresh and Smith. 2004). Axenization was conducted after the isolates have been incubated for 2 to 3 days. Isolation of solubilized antigen from symptomatic and asymptomatic cultures The axenic B. hominis species were collected using Ficoll-Paque density gradient centrifugation method and the harvested parasites were resuspended in basal Jones medium without the addition of heat-inactivated horse serum. Then, the harvested organisms were lysed by sonication method and the homogenate was incubated at 4 °C, overnight. The homogenate was centrifuged at 13000xg for 15 minutes at 4 °C after the overnight incubation. The supernatant which contains the solubilized antigen of B. hominis was filter-sterilized and the protein concentration of the antigen was determined using Bradford assay (Bio-Rad, USA). Subtyping of Blastocystis isolates from symptomatic and asymptomatic cultures Genomic DNA Extraction: The genomic DNA of symptomatic and asymptomatic isolates of Blastocystis hominis were extracted from the 3 to 4 days old culture with QIAmp DNA stool mini kit (Qiagen, Hilden, Germany) according to the manufacturers protocol. DNA Purity and Concentration: The genomic DNA yields were estimated using measurement of absorbance at 260nm and 280nm using a spectrophotometer. The ratio of absorbance at 260nm to 280nm determines the purity of the genomic DNA yielded. According to the manufacturers protocol, the ratio of 1.7-1.9 indicates that the DNA yields are pure. PCR Typing by STS Primers: The genotype of B. hominis was determined by using PCR amplification with subtype-specific sequence tagged site (STS) primers (SB83, SB155, SB227, SB332, SB340, SB336, and SB337). The PCR reaction mixtures (20  µl of total volume) consisted of PCR buffer, 2.5 U/ µl of Taq DNA polymerase (Fermantas, SB38), 1.5 mM MgCl2, 1.25  µM of dNTPs (Fermantas, RO191), 0.5 pmol of forward primer, 0.5 pmol of reverse primer, and 1.0  µl of the DNA samples. In addition, the PCR conditions were set as follows: one cycle denaturing at 94 °C for 3 min, 30 cycles including denaturing at 94 °C for 30 seconds, annealing at 56.3 °C for 30 seconds, extending at 72 °C for 60 s, , and additional cycle with a 10 min chain elongation at 72 °C. The PCR products obtained were then electrophoresed in 1.5% agarose gel with 1X Tris-boric-EDTA (TBE) buffer. The fragments of the DNA were visualized using UV illuminator under UV light. The fragment sizes of the genomic DNA were confirmed with bands of a DNA ladder (50-1,000 bp DNA markers, Fermentas). Cultivation and collection of HCT116 Human colorectal carcinoma cell line, HCT116, was cultured in 25 cm ³ culture flask containing 5 ml of RPMI 1640 growth medium, supplemented with 5% of FBS, 2mM L-glutamine, 100 U/ml penicillin-streptomycin, and 2.5 ÃŽ ¼g/ml fungizone. The HCT116 cell line was then incubated in a CO2 incubator set at 100% humidity, atmosphere containing 5% of CO2 and a temperature of 37 °C. Prior to the introduction of antigen from Blastocystis hominis isolates, the cells were harvested from the substratum of the culture flask by using 0.25% trypsin-EDTA. The harvested cells were then washed with phosphate-buffered saline (PBS) thrice and resuspended in RPMI 1640 growth medium before introducing the solubilized antigen of Blastocystis hominis from symptomatic and asymptomatic isolates. Isolation of peripheral blood mononuclear cells (PBMCs) PBMCs were collected from fresh human blood sample (12 ml) in sterile EDTA tubes. The PBMCs were then isolated from the blood sample by using Histopaque ®-1077 (Sigma-Aldrich, USA) according to the density gradient centrifugation method (Boyum. 1974). The isolated PBMCs were then washed with PBS thrice and resuspended in 5 ml of RPMI 1640 growth medium, supplemented with 10% of FBS, 2mM L-glutamine, 100 U/ml penicillin-streptomycin, and 2.5 ÃŽ ¼g/ml fungizone in a 25 cm ³ culture flask prior to the introduction of solubilized antigen of Blastocystis hominis isolates. Introduction of the solubilized symptomatic and asymptomatic derived Blasto antigen into PBMCs and colorectal cancer cell line, HCT116 Harvested HCT116 cells (1 x 103 cells per well) in 100 ÃŽ ¼l of RPMI growth medium with 5% FBS and freshly isolated PBMCs (5 x 104 cells per well) in 100 ÃŽ ¼l of RPMI growth medium with 10% FBS were seeded into 96 well plates. After the overnight incubation in a CO2 incubator containing 5% CO2 at 37 °C, Blasto-antigen of B. hominis from symptomatic and asymptomatic individuals at final concentration ranging from 0.001 to 10ÃŽ ¼g/ml was added to each well containing the PBMCs and HCT116 cells and were further incubated for 48 hours. Then, the cell proliferation/viability was measured using the MTT assay (Mosmann. 1983). Quantitative Real-time Reverse Transcription Polymerase Chain Reaction (rtRT-PCR) Concentration of symptomatic and asymptomatic Blasto-antigen that yields the optimal proliferation and inhibition in the preliminary tests (MTT assay) was used to introduce into each culture flask containing PBMCs and HCT 116 cells respectively. For controls, PBS was introduced into both types of cells. After 48 hours of incubation, the RNA was isolated from the PBMCs and HCT 116 cells using Ambion RNAqueous Micro Kit (Ambion, CA, USA). The purified RNA obtained was then used to synthesize complementary DNA (cDNA) using High-Capacity RNA-to-cDNA kit (Applied Biosystems, USA) by the PCR method. Finally, Real-time reverse transcription PCR analysis was performed using inventoried primers (TaqMan ® Gene Expression Assays, Applied Biosystems). In this study, the genes of interest were IL-6, IL-8, tumour necrosis factor alpha (TNF-ÃŽ ±), nuclear factor kappa light chain enhancer of activated B cells (NF-ÃŽ ºB), interferon gamma (IFN-ÃŽ ³), protein 53 (p53), transforming growth factor be ta (TGF-ÃŽ ²), and cathepsin B (CTSB). The PCR reaction will be prepared according to the protocol that is generated by StepOneTM Software v2.0. Statistical Analysis In order to analyze the real-time RT-PCR gene expressions, the à ¢Ã‹â€ Ã¢â‚¬  CT value of the treated samples with symptomatic and asymptomatic Blasto-antigen was compared against the non-treated sample (control with PBS) using the Students t test. A P value of 0.05 was considered to be the minimum threshold of significance. RESULTS AND DISCUSSIONS In the present study, the symptomatic and asymptomatic isolates were genotyped by PCR amplification using the well-known seven sets of STS primers. The extracted DNA of symptomatic isolate showed positive amplification with SB83 and thus determined as subtype 1 (Yoshikawa et al. 2004). Nevertheless, the extracted DNA of asymptomatic isolate did not show any positive amplification with all the seven sets of STS primers that was used in this study. Hence, it is postulated that the asymptomatic isolate may be subtype 8 to 13 which was not amplified by the STS primers used in this study (Dominguez-Mà ¡rquez et al. 2009; Eroglu et al. 2009; Jones et al. 2009; Hussein et al. 2008; Tan et al. 2008). Molecular phylogenetic analysis need to be carried out in order to further characterized the subtype of asymptomatic isolate used in this study (Noel et al. 2005; Stensvold et al. 2007; Yoshikawa et al. 2007). In this study, the Blasto-Ag isolated from both symptomatic as well as asymptomatic isolates have caused an inhibition of PBMC cells proliferation which leads to the speculation that the Blasto-Ag may have caused apoptosis in the immune cells to prevent the propagation of immune cells to combat with the Blastocystis infection. The symptomatic isolate has caused a greater inhibition of PBMCs as compare to asymptomatic isolates. However, the Blasto-Ag mediated PBMCs cell inhibition is contrast to our previous report (Chandramathi et al. 2010). Our previous study showed that Blasto-Ag caused stimulation of PBMCs cell proliferation (Chandramathi et al. 2010). However, the molecular genotyping of the B. hominis isolates used in that study was not carried out. Past studies have reported that there may be a correlation between different B. hominis subtypes on the pathogenesis in human hosts (Dominguez-Mà ¡rquez et al. 2009; Eroglu et al. 2009; Jones et al. 2009; Hussein et al. 2008; Tan et al. 2008). This contrasting result observed in the cell viability of PBMCs may suggest the different subtypes of B. hominis may give different effects on the immune cells. Hence, further investigation is required to investigate the potential pathogenesis of different B. hominis subtypes. In the current study, increase in cell proliferation has been observed in colorectal carcinoma cell line, HCT116 cells upon exposure to antigen from symptomatic as well as asymptomatic isolates. HCT116 cells have shown significantly higher increase in cell proliferation stimulated by symptomatic Blasto-Ag as compared to asymptomatic antigen. The increase in the cell proliferation may further suggest that Blastocystis infection could facilitate the growth of colorectal cancer cells (Chandramathi et al. 2010). It is evident from the present study that symptomatic Blasto-Ag is more pathogenic as compared to asymptomatic Blasto-Ag. Hence, it may be essential to treat the Blastocystis infection in symptomatic colorectal cancer patients for better prognosis. On the other hand, a diverse pattern of cytokine, nuclear transcription factor as well as apoptotic gene expressions were observed upon the stimulation of HCT116 cells and PBMCs by symptomatic and asymptomatic derived Blasto-Ag. In the present study a noticeable up-regulation of Th1 (IFN-ÃŽ ³ and TNF-ÃŽ ±) and Th2 (IL6, IL8 and TGF-ÃŽ ²) cytokines was observed in HCT116 following exposure to symptomatic Blasto-Ag may lead to the speculation that B. hominis has activated the cellular and humoral immune responses in clearing the Blastocystis infection. This is accordance to the previous study that extracellular parasites such as B. hominis would be more effective in counteracted by a combination of Th1 and Th2 cytokines (Daugelat et al. 1996). Nevertheless, a more significant up-regulation of Th2 cytokines as compared to Th1 cytokines observed in HCT116 upon exposure to symptomatic and asymptomatic Blasto-Ag may lead to the postulation that symptomatic Blasto-Ag may resulting in a Th2- dominated responses and has the potential in weakening the cellular immune response, allowing the progression and growth of an existing tumour cells. On the other hand, HCT116 exposed to symptomatic Blasto-Ag has resulted in a more noticeable up-regulation of Th2 cytokines then asymptomatic Blasto-Ag which may lead to the speculation that asymptomatic Blasto-Ag is less pathogenic than symptomatic derived Blasto-Ag. Furthermore, the up-regulation of TGF-ÃŽ ² in the colorectal cancer cells has the potential role of anti-inflammatory and resulting in host immunosuppression by inhibiting the cell mediated immune responses against the tumour cells (Zou, 2005; Seruga et al. 2008). In addition, it is widely-accepted that humoral immunity is activated by extracellular microbes such as bacteria (Romagnani, 1996). B. hominis, being an extracellular allergen, has triggered the activation of host humoral immune responses which is evident by the significant up-regulation of Th2 cytokines in the PBMCs upon exposure to symptomatic and asymptomatic Blasto-Ag. Moreover, this results can be supported by the recent finding which shown an elevation of IL6 and IL8 level in the monocytes of healthy volunteers exposed to Leishmania infection (Menezes et al. 2008). In spite of that, an interesting finding has been observed in PBMCs stimulated by symptomatic and asymptomatic Blasto-Ag, besides activating the humoral immune responses, PBMCs exposed by Blasto-Ag has also triggered the activation of cellular mediated immune response characterized by Th1 cytokines. As described earlier, extracellular parasites may be more effective counteracted by a combination of Th1 and Th2 cytoki nes mediated immune responses (Daugelat et al. 1996). However, the activation of cellular mediated immune responses which is reflected by a significant up-regulation of TNF-ÃŽ ± and IFN-ÃŽ ³ may lead to the speculation that Blasto-Ag isolated from symptomatic as well as asymptomatic individual has the ability in causing extensive inflammatory damage in the host tissue as a result of macrophage mediated responses towards the parasitic infections. In addition, an up-regulation of NF-ÃŽ ºB and pro-inflammatory cytokines IL6 and IL8 was observed in HCT116 stimulated by symptomatic Blasto-Ag. Furthermore, these findings were also observed in PBMCs stimulated by symptomatic and asymptomatic Blasto-Ag. Nevertheless, HCT116 exposed to asymptomatic Blasto-Ag has exhibited an insignificant down-regulation of NF-à Ã‚ ºB gene expression which then leads to a significant lower expression in IL6 and IL8 as compared to HCT116 stimulated by symptomatic Blasto-Ag. Although the previous reports by Chandramathi et al. 2010 has stated the potential role of asymptomatic Blasto-Ag in causing the up-regulation of NF-à Ã‚ ºB and pro-inflammatory cytokines IL6 and IL8, however, the similar trend was not observed in the current study. As described earlier, different Blastocystis subtypes may have different effects on HCT116 cells as well as causing different gene expression profile in HCT116. To further characterize the gene expression profiles a s well as pathogenic potential of each subtype on HCT116 and PBMCs, more samples on different subtypes need to be recruited for the future study. Apart from this, NF-à Ã‚ ºB is also related to the apoptotic mediator genes namely p53 and CTSB. NF-à Ã‚ ºB has been postulated to play a role in inhibiting the apoptosis; hence it is hypothesized that the down-regulation of NF-à Ã‚ ºB gene expression will lead to the up-regulation of p53 and vice versa (Baldwin, 2001; Chwieralski et al. 2006). Although the recent study has suggested that CTSB may contribute to the cell apoptosis, however the studies have only reported on CTSB-mediated apoptosis in breast cancer cells as well as hepatocytes apoptosis in fulminant hepatic failure (Sandes et al, 2007; Yan et al. 2009). Nevertheless, the possibility of CTSB participation in the apoptosis of colorectal cancer cells has not been investigated. Moreover, there are a number of studies have shown that over expression of CTSB mRNA and elevation of its protein activity have been associated with the invasive and metastasis properties of various cancers (Campo et al. 1994; Yan et al, 1998; Hirai et al. 1999; Sanjeeva et al. 2001). The similar findings were observed in the current study where the HCT116 cells stimulated by symptomatic as well as asymptomatic Blasto-Ag have caused a significant up-regulation of CTSB. The over expression of CTSB in HCT116 cells stimulated by symptomatic and asymptomatic Blasto-Ag may implicate the potential of Blasto-Ag in causing the invasive and metastasis of colorectal cancer (Campo et al. 1994; Yan et al. 1998; Dora et al. 2003). In the present study, the insignificant down-regulation of p53 gene expressions observed in PBMCs may indicates that the symptomatic and asymptomatic Blasto-Ag may have prevented the PBMC cells to undergo the apoptosis process. The observation of p53 gene expressions obtained was contrary to the expectation. This may be due to the latent effects of the host immunity to down-regulate the apoptotic mediator gene in order to combat with the invasive B. hominis as well as to prevent persistent inflammation which can lead to carcinogenesis. Although the down-regulation pattern was observed, it does not causing a significant effect (0.95 and 0.96 fold decrease respectively). In contrast to PBMCs, stimulation of symptomatic Blasto-Ag in HCT116 has insignificantly down-regulated the gene expressions of p53. The insignificant down-regulation observed in HCT116 stimulated by symptomatic Blasto-Ag may be explained that it could be the reason that HCT116 cells to have low level of NF-à Ã‚ ºB gene expression level (1.34 fold increase). Nevertheless, the down-regulation observed may further suggest that symptomatic Blasto-Ag may has enhance the proliferation and progression of existing tumour besides causing an extensive release of pro-inflammatory cytokines. On the other hand, an interesting finding was observed in HCT116 exposed to asymptomatic Blasto-Ag, instead of causing down-regulation of p53 gene expression, asymptomatic Blasto-Ag has caused an up-regulation of p53 (1.09 fold increase) in HCT116. The findings and observations obtained from the current study are still far to characterize the mechanism of the potential pathogenic role of B. hominis in PBMCs as well as HCT116 cells. However, the current study was able to give an overview of the potential pathogenesis of symptomatic and asymptomatic derived Blasto-Ag in HCT116 and PBMCs. Moreover, the findings also suggest that symptomatic derived Blasto-Ag is more pathogenic as compare to asymptomatic Blasto-Ag. More studies still need to be done on the association of this emerging parasite on the association with the colorectal cancer. CONCLUSION The MTT cell proliferative assay and gene expression profile in this study has shown that the solubilized antigen isolated from symptomatic and asymptomatic individuals, at a particular concentration, could facilitate the proliferation and growth of colorectal carcinoma while having the ability to induce apoptosis on PBMCs immune cells. Moreover, the cell proliferative assay also successfully reported that antigen isolated from symptomatic individual is more pathogenic as compare to asymptomatic isolates, as it causes a significantly higher increase in cell proliferation of HCT116 colorectal carcinoma cell lines and a significantly higher increase in inhibition of PBMCs immune cells. Moreover, symptomatic isolate of B. hominis has exhibited a more evident increase in the gene expression of Th1 and Th2 cyokines. Hence, there is a vital need to screen colorectal cancer patients for B. hominis infection.

Child Observation Essay -- Child Development Project

Leroy is a 2nd grade African American student at Martin Luther King Elementary School. He is 8 years old and lives with his mother and two older brothers. His favorite school subject is math. He likes to play video games and basketball, especially when he is playing with his friends. Leroy’s favorite holiday is Christmas, for he gets to visit his grandma and enjoy all the delicious foods that his mom and grandma cook. When asked where would he go if he can go anywhere, he responded with Skate Park. He likes to skate around the park with his brothers. Leroy wishes to be like his older brother when he grows up. Leroy is a competitive student even among his friends. He likes to read at the same table as his friends or in the same room with them. When his friends are present, Leroy is more cooperative. He follows instructions, and he puts more effort into his readings. He wants to perform better than his friends; however, once he begins to struggle, Leroy loses his motivation to succeed in the task. According to Svinicki (2005), Leroy’s behavior and attitudes portray a student with a performance goal. To be more specific, a student with a performance approach goal, for Leroy’s main interest is to appear competent. The main reasons for the effort he puts in are to surpass his peers in order to receive recognition and attention. After determining Leroy’s goal orientation, my goal was to encourage him to shift from performance approach goal to achievement goal. I believe shifting Leroy’s goal orientation will benefit Leroy in numerous ways. He will be intrinsically motivated to read, which will increase his engagement in the reading games that we perform together. When he is engaged in learning the contents for himself, h... ...uring challenging tasks. In order to encourage Leroy to shift his goal orientation from performance approach goal to mastery goal, a variety of supports may need to be provided. Leroy needs to learn in an environment that minimizes competition and supports failures as learning opportunities. He needs to witness his accomplishments and recognizes that it is his effort and not ability or inability that resulted in his success. It may take some time for Leroy to shift his goal orientation from a performance approach to an achievement goal, but I believe with the right support and encouragement, Leroy will be able to develop a mastery goal. Works Cited Dweck, C.S. (2007). The perils and promises of praise. Educational Leadership, 65(2), 34-39. Svinicki, M. (2005, February). Student goal orientation, motivation, and learning. Idea paper #41, Idea Center.

Interpretations of American History Essay

There has been a notable change in the American History from the last 400 years until now. At the beginning the American History the subjects that the historians interpreted were politics, diplomacy and war. The only people who could do the interpreting were only white male Americans. The contemporary American historians wrote nearly about everything that affected everybody. This times were very different from what they are now, when you read history, you are reading the historian’s point of view or encounter with the world. The historians were fully dedicated to this, they spend hours, days, years of their life’s to prove the reality comes away. They were characterized by nationality or their school of thought. The historians can be Jeffersonian liberal, nationalist, progressive, feminist or postmodernist; for example, Perry Miller in the twentieth century American liberalism, Kenneth Stampp’s was engaged with the civil rights movement, Kathryn Sklar’s ideas were feminist (p. 72). In those times everything was like they African American people couldn’t be historians, because they only accepted white male Americans, also they were male chauvinist, women couldn’t be historians and not only that, they were excluded from everything, they didn’t have the right to vote, to be in the politics, work, they Jimenez 2 could not do anything. History is successful when it tells you how things where, the only thing that could take you there was the imagination, but now, with all the technology that we have, we even can make a picture or a sketch of how the things were. basing on the things that had been found also. One of the things that the historians did, they constantly criticize, correct and supplement other historian point of view, they get closer to the truth arguing with one another. Historiography reminds you that history is not a closed book, is a reminder that there is always something to argue in history, it make us think or see what was before everything, the changes that have been in the growth of America, politics changes, racial justice, sex, differences in the society, education, labor. There has been a notorious change or evolution on how America has change. There are four stages in which the writing of American history has passed: the providential, the rationalist, the nationalist and the professional. The providential history in the United States from the seventeenth and eighteenth, ministers and magistrates and wrote a made of form of providential history. The puritans who settled in the United States believed that they were chosen by god that led to the form of their history to a holy chronicle one. Puritans used this form of writing for all men anybody that believed in god. The rationalism, they were very different with the providential because they didn’t believed that they were only chosen by god, they believed that opinions or actions should act on reason and knowledge not on their religious belief. They were ruled by the natural law was the idea of Newton, Locke, and the French philosophers (p. 75). The rationalist Jimenez 3 historians were the educated class in the colonies, the new story they told was of progress and reason. most of the historians of the eighteenth century were layer-politicians, planter-aristocrats, merchants or professionals. Tomas Jefferson was influenced by this movement and later on write a book named Notes on the State of Virginia in 1785. This book allow to men to discover and pursue their destiny and believed that they were free. After that, no one was free from slavery. This proves that the history during the rationalism the politicians only tells you what you want to hear, like right now it happens the same, everything was and is only about wealth, power, rights. In nationalist history changed the fact that in this stage the women contributed and argue in theirs perspectives or points of view. historians of women rewrote the story of America from the beginning to the recent past. They did not give women a place in the existing narratives, they just reconceived whole fields of history. An example of the women historians were Hanna Adams, Susanna Rowson, Elizabeth Peabody, and Emma Willard (p. 76) The professional historians started by the middles class that became educated through college or university which became commonly to only white males. History became a profession and the only way to access was to practice it and be at a advanced level in the subject. Their high opinion on distinguish scientifically and prove the truth from a romantic notion. In this time we can see through what has passed American History and learned about the different changes that our history has had, and also this has help to have America Jimenez 4 how is it now. Right now we see the thing different, for example, women can vote, women can do a lot more things that they couldn’t in the past, there is no slavery, everyone is free and follow their dreams and conquer their goals and that’s a big change.